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The recent breakthrough in stem cell research paved the way for new developments in cell therapy and regenerative medicine. The culture media is a critical element for maintaining healthy & proliferating stem cells in culture; and working with a highly pure and defined stem cell medium is key for regenerative medicine applications. Several types of culture media are available for use in stem cell research:

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Fig 1. Types of culture media for stem cell research

The extensive usage of animal derived (xenogeneic) reagents and growth factors have raised the concern for risk of contamination, immunogenicity, high cost, traceability and also considerable ethical concerns of the 3Rs (replacement, reduction, refinement). These xenogeneic products are of undefined qualitative and quantitative composition, which are added to prepare culture media for research studies. Thus, xeno-free and animal component free culture conditions are desirable.

The method of choice would be the supplementation of basal culture media with growth factors of non-animal origin (NAO) or ACF CD media that support cell growth, proliferation and differentiation.

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Fig 2.Representation of how ACF CD media can reduce the risk of contamination and undefined composition

Richcore is deeply committed to provide NAO recombinant growth factors and reagents for stem cell, regenerative medicine research & biomanufacturing; which are reliable, safer, cost-effective & highly pure. These products are used in production of Animal Component Free Chemically Defined (ACF CD) media.

Fig 2. Represents how Richcore’s NAO recombinant products can reduce the risk of safety & complexity by incorporating into ACF CD culture media formulations for stem cell & regenerative medicine research. The most important attribute of Richcore’s NAO products compared to other NAO products is, Richcore provides these products with high quality and at less or almost comparable price to serum derived components; which makes it quite viable as a cost effective alternative in stem cell research and future scale up.

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References

Serum-free cell culture: the serum-free media interactive online database. Brunner D et al. ALTEX. 2010;27(1):53-62

A plea to reduce or replace fetal bovine serum in cell culture media. Gstraunthaler G et al. Cytotechnology. 2013 Oct;65(5):791-3

Akopian, V. et al. (2010) Comparison of defined culture systems for feeder cell free propagation of human embryonic stem cells. In Vitro Cellular and

Developmental Biology. 46(3): 247-258. Alternatives to the use of fetal bovine serum: serum-free cell culture. Gstraunthaler G. ALTEX. 2003;20(4):275-81

Rodin, S. et al. (2014) Clonal culturing of human embryonic stem cells on laminin-521/E-cadherin matrix in defined and xeno-free environment. Nature Communications. 5:3195.

Azouna, N. B. et al. (2012) Phenotypical and functional characteristics of mesenchymal stem cells from bone marrow: comparison of culture using different media supplemented with human platelet lysate or fetal bovine serum. Stem Cell Research & Therapy. 3:6

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